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pseudotyped retrovirus with pumvc3  (Addgene inc)


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    Structured Review

    Addgene inc pseudotyped retrovirus with pumvc3
    Pseudotyped Retrovirus With Pumvc3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pseudotyped+retrovirus+with+pumvc3/pUMVC+(Plasmid+%238449)/pmc12005928-300-57-61
    Average 95 stars, based on 172 article reviews
    pseudotyped retrovirus with pumvc3 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Construct:

    Article Title: The Far Upstream Element Binding Protein 3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells
    Article Snippet: FUBP3-HA-WT was initially constructed in a pcDNA3.1 vector and was then used as a template for deletions (Table S1). .. FUBP3 constructs were then inserted in a pMSCV-IRES-mcherry vector (Addgene, #52114) and packaged with pCVM-VSV-G (Addgene #8454) and CMV intron (a gift from Greg Towers, UCL) for overexpression experiments. pMKO.1-puro (Addgene, #8452) was used for shRNA (targeting CCNT1, FUBP1, FUBP2, FUBP3, and CD8B, Table S1) cloning and was packaged in HEK293T cells for pseudotyped retrovirus with pUMVC3 (Addgene #8449) and pCVM-VSV-G (Addgene #8454). .. LMPd-ametrine vector shRNAmirs (targeting FUBP3 and CD19, Table S1) were packaged for pseudotyped retrovirus in HEK293T cells with pCVM-VSV-G (Addgene #8454) and CMVintron (a gift from Dr. François-Loïc Cosset). pFI-Tat(86)-FLAGWT, pFI-Tat(86)-FLAG-BRM, and other Tat mutation/deletions constructs were generated as previously described.35,53 Primary cell culture and transduction PBMCs were first separated from blood from three healthy donors (LifeSouth Community Blood Center) using Ficoll-Paque Plus (Cytiva, #17144002).

    Article Title: FUBP3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells
    Article Snippet: FUBP3-HA-WT was initially constructed in a pcDNA3.1 vector and was then used as a template for deletions ( ). .. FUBP3 constructs were then inserted in a pMSCV-IRES-mcherry vector (Addgene, #52114) and packaged with pCVM-VSV-G (Addgene #8454) and CMV intron (a gift from Greg Towers, UCL) for overexpression experiments. pMKO.1-puro (Addgene, #8452) was used for shRNA (targeting CCNT1 , FUBP1 , FUBP2 , FUBP3 , and CD8B , ) cloning and was packaged in HEK293T cells for pseudotyped retrovirus with pUMVC3 (Addgene #8449) and pCVM-VSV-G (Addgene #8454). .. LMPd-ametrine vector shRNAmirs (targeting FUBP3 and CD19 , ) were packaged for pseudotyped retrovirus in HEK293T cells with pCVM-VSV-G (Addgene #8454) and CMV-intron (a gift from Dr. François-Loïc Cosset). pFI-Tat(86)-FLAG-WT, pFI-Tat(86)-FLAG-BRM, and other Tat mutation/deletions constructs were generated as previously described.,

    Over Expression:

    Article Title: The Far Upstream Element Binding Protein 3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells
    Article Snippet: FUBP3-HA-WT was initially constructed in a pcDNA3.1 vector and was then used as a template for deletions (Table S1). .. FUBP3 constructs were then inserted in a pMSCV-IRES-mcherry vector (Addgene, #52114) and packaged with pCVM-VSV-G (Addgene #8454) and CMV intron (a gift from Greg Towers, UCL) for overexpression experiments. pMKO.1-puro (Addgene, #8452) was used for shRNA (targeting CCNT1, FUBP1, FUBP2, FUBP3, and CD8B, Table S1) cloning and was packaged in HEK293T cells for pseudotyped retrovirus with pUMVC3 (Addgene #8449) and pCVM-VSV-G (Addgene #8454). .. LMPd-ametrine vector shRNAmirs (targeting FUBP3 and CD19, Table S1) were packaged for pseudotyped retrovirus in HEK293T cells with pCVM-VSV-G (Addgene #8454) and CMVintron (a gift from Dr. François-Loïc Cosset). pFI-Tat(86)-FLAGWT, pFI-Tat(86)-FLAG-BRM, and other Tat mutation/deletions constructs were generated as previously described.35,53 Primary cell culture and transduction PBMCs were first separated from blood from three healthy donors (LifeSouth Community Blood Center) using Ficoll-Paque Plus (Cytiva, #17144002).

    Article Title: FUBP3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells
    Article Snippet: FUBP3-HA-WT was initially constructed in a pcDNA3.1 vector and was then used as a template for deletions ( ). .. FUBP3 constructs were then inserted in a pMSCV-IRES-mcherry vector (Addgene, #52114) and packaged with pCVM-VSV-G (Addgene #8454) and CMV intron (a gift from Greg Towers, UCL) for overexpression experiments. pMKO.1-puro (Addgene, #8452) was used for shRNA (targeting CCNT1 , FUBP1 , FUBP2 , FUBP3 , and CD8B , ) cloning and was packaged in HEK293T cells for pseudotyped retrovirus with pUMVC3 (Addgene #8449) and pCVM-VSV-G (Addgene #8454). .. LMPd-ametrine vector shRNAmirs (targeting FUBP3 and CD19 , ) were packaged for pseudotyped retrovirus in HEK293T cells with pCVM-VSV-G (Addgene #8454) and CMV-intron (a gift from Dr. François-Loïc Cosset). pFI-Tat(86)-FLAG-WT, pFI-Tat(86)-FLAG-BRM, and other Tat mutation/deletions constructs were generated as previously described.,

    shRNA:

    Article Title: The Far Upstream Element Binding Protein 3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells
    Article Snippet: FUBP3-HA-WT was initially constructed in a pcDNA3.1 vector and was then used as a template for deletions (Table S1). .. FUBP3 constructs were then inserted in a pMSCV-IRES-mcherry vector (Addgene, #52114) and packaged with pCVM-VSV-G (Addgene #8454) and CMV intron (a gift from Greg Towers, UCL) for overexpression experiments. pMKO.1-puro (Addgene, #8452) was used for shRNA (targeting CCNT1, FUBP1, FUBP2, FUBP3, and CD8B, Table S1) cloning and was packaged in HEK293T cells for pseudotyped retrovirus with pUMVC3 (Addgene #8449) and pCVM-VSV-G (Addgene #8454). .. LMPd-ametrine vector shRNAmirs (targeting FUBP3 and CD19, Table S1) were packaged for pseudotyped retrovirus in HEK293T cells with pCVM-VSV-G (Addgene #8454) and CMVintron (a gift from Dr. François-Loïc Cosset). pFI-Tat(86)-FLAGWT, pFI-Tat(86)-FLAG-BRM, and other Tat mutation/deletions constructs were generated as previously described.35,53 Primary cell culture and transduction PBMCs were first separated from blood from three healthy donors (LifeSouth Community Blood Center) using Ficoll-Paque Plus (Cytiva, #17144002).

    Article Title: FUBP3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells
    Article Snippet: FUBP3-HA-WT was initially constructed in a pcDNA3.1 vector and was then used as a template for deletions ( ). .. FUBP3 constructs were then inserted in a pMSCV-IRES-mcherry vector (Addgene, #52114) and packaged with pCVM-VSV-G (Addgene #8454) and CMV intron (a gift from Greg Towers, UCL) for overexpression experiments. pMKO.1-puro (Addgene, #8452) was used for shRNA (targeting CCNT1 , FUBP1 , FUBP2 , FUBP3 , and CD8B , ) cloning and was packaged in HEK293T cells for pseudotyped retrovirus with pUMVC3 (Addgene #8449) and pCVM-VSV-G (Addgene #8454). .. LMPd-ametrine vector shRNAmirs (targeting FUBP3 and CD19 , ) were packaged for pseudotyped retrovirus in HEK293T cells with pCVM-VSV-G (Addgene #8454) and CMV-intron (a gift from Dr. François-Loïc Cosset). pFI-Tat(86)-FLAG-WT, pFI-Tat(86)-FLAG-BRM, and other Tat mutation/deletions constructs were generated as previously described.,

    Cloning:

    Article Title: The Far Upstream Element Binding Protein 3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells
    Article Snippet: FUBP3-HA-WT was initially constructed in a pcDNA3.1 vector and was then used as a template for deletions (Table S1). .. FUBP3 constructs were then inserted in a pMSCV-IRES-mcherry vector (Addgene, #52114) and packaged with pCVM-VSV-G (Addgene #8454) and CMV intron (a gift from Greg Towers, UCL) for overexpression experiments. pMKO.1-puro (Addgene, #8452) was used for shRNA (targeting CCNT1, FUBP1, FUBP2, FUBP3, and CD8B, Table S1) cloning and was packaged in HEK293T cells for pseudotyped retrovirus with pUMVC3 (Addgene #8449) and pCVM-VSV-G (Addgene #8454). .. LMPd-ametrine vector shRNAmirs (targeting FUBP3 and CD19, Table S1) were packaged for pseudotyped retrovirus in HEK293T cells with pCVM-VSV-G (Addgene #8454) and CMVintron (a gift from Dr. François-Loïc Cosset). pFI-Tat(86)-FLAGWT, pFI-Tat(86)-FLAG-BRM, and other Tat mutation/deletions constructs were generated as previously described.35,53 Primary cell culture and transduction PBMCs were first separated from blood from three healthy donors (LifeSouth Community Blood Center) using Ficoll-Paque Plus (Cytiva, #17144002).

    Article Title: FUBP3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells
    Article Snippet: FUBP3-HA-WT was initially constructed in a pcDNA3.1 vector and was then used as a template for deletions ( ). .. FUBP3 constructs were then inserted in a pMSCV-IRES-mcherry vector (Addgene, #52114) and packaged with pCVM-VSV-G (Addgene #8454) and CMV intron (a gift from Greg Towers, UCL) for overexpression experiments. pMKO.1-puro (Addgene, #8452) was used for shRNA (targeting CCNT1 , FUBP1 , FUBP2 , FUBP3 , and CD8B , ) cloning and was packaged in HEK293T cells for pseudotyped retrovirus with pUMVC3 (Addgene #8449) and pCVM-VSV-G (Addgene #8454). .. LMPd-ametrine vector shRNAmirs (targeting FUBP3 and CD19 , ) were packaged for pseudotyped retrovirus in HEK293T cells with pCVM-VSV-G (Addgene #8454) and CMV-intron (a gift from Dr. François-Loïc Cosset). pFI-Tat(86)-FLAG-WT, pFI-Tat(86)-FLAG-BRM, and other Tat mutation/deletions constructs were generated as previously described.,



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    Addgene inc pseudotyped retrovirus with pumvc3
    Pseudotyped Retrovirus With Pumvc3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pseudotyped+retrovirus+with+pumvc3/pUMVC+(Plasmid+%238449)/pmc12005928-300-57-61
    Average 95 stars, based on 1 article reviews
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